Ice Nucleation Active bacteria in Mount Lawu forest, Indonesia: 1. Isolation and estimation of bacterial populations on lichens

Abstract. Fu’adah K, Sari SL, Susilowati A. 2017. Ice Nucleation Active bacteria in Mount Lawu forest, Indonesia: 1. Isolation and estimation of bacterial populations on lichens. Asian J For 1: 83-91. (INA) bacterias cause frost injury plants. These are also predicted to play an important role bioprecipitation, cloud formation, rain. Most INA studies conducted subtropical areas. Therefore, from tropical areas, especially Indonesia, need be conducted. This study aimed isolate determine the number lichenes. samples lichenes were taken hiking pathway Cemoro Sewu, Java, at ±2.200, ±2.400, ±2.600 m asl. isolated by spread plate method NA medium with 2.5 % glycerol King’s B medium. A tube nucleation test determined ice activity. multiple-tube estimated bacteria. The data analyzed descriptively based colony morphology, cell biochemical tests, analysis numbers result showed that 7 isolates Parmelia sp. altitude 2.532 asl (station 2) have been known as bacterias. was 5 x 104/g, which very low.